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Aging-associated subpopulations of human CD8+ T-lymphocytes identified by their CD28 and CD57 phenotypes

Tijdschriftbijdrage - Tijdschriftartikel

BACKGROUND: During organismal aging, human T-cells shift towards less functional phenotypes, often called senescent cells. As these cells have not been well characterized, we aimed to relate surface markers of human T-cell senescence with characteristics of in vitro cellular aging and to further characterize these cells.

METHODS: We identified, by flow cytometry, subpopulations of CD8+ T-cells based on CD57 and CD28 expression, and tested them for some markers of cellular senescence, apoptosis, differentiation and homing.

RESULTS: Elderly persons presented significantly higher proportions not only of CD28-CD57+, but also of CD28+CD57+ cells. CD28+CD57+ cells had the highest expression of p16, p21, Bcl-2, CD95, CD45RO, CCR5 and PD-1, thereby arguing in favor of a senescent phenotype.

CONCLUSION: Among CD8+ T-lymphocytes, CD28+CD57+ cells represent a subset with some senescent features that are distinct from the CD28-CD57+ cells.

Tijdschrift: Archives of Gerontology & Geriatrics
ISSN: 0167-4943
Issue: 3
Volume: 61
Pagina's: 494-502
Jaar van publicatie:2015
  • VABB Id: c:vabb:395263
  • ORCID: /0000-0002-6820-9586/work/75885592
  • ORCID: /0000-0002-8853-6031/work/58609269
  • WoS Id: 000368012900026
  • Scopus Id: 84943449487
CSS-citation score:2