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Contribution of postnatally formed small beta cell aggregates to functional beta cell mass in adult rat pancreas
Tijdschriftbijdrage - Tijdschriftartikel
AIMS/HYPOTHESIS: Neogenesis of beta cells and their clustering to small aggregates is a key process in prenatal development of beta cell mass. We investigated the contribution of postnatally formed small aggregates to functional beta cell mass in adult rats.
METHODS: Conditions were defined for (1) counting total beta cell number in pancreases with relative error of
RESULTS: Pancreases of 10-week-old male Wistar rats contained 2.8 +/- 0.2 x 10(6) beta cells, of which >90% was generated postnatally, involving: (1) neo-formation of 30,000 aggregates with diameter 200 mum, their beta cells exhibited a higher basal insulin content that was also resistant to glibenclamide-induced degranulation. The pool of Ki67-positive beta cells was sixfold larger than at birth and distributed over all aggregate sizes.
CONCLUSIONS/INTERPRETATION: We describe a method for in situ counting of beta cell numbers and subpopulations with low relative error. In adult rats, >90% of beta cells and beta cell aggregates are formed after birth. Aggregates 200 mum, which are selected for isolated islet studies. Their topographic and functional properties contribute to the functional heterogeneity of the beta cell population; their growth to larger aggregates with characteristic beta cell functions may serve future metabolic needs.
METHODS: Conditions were defined for (1) counting total beta cell number in pancreases with relative error of
RESULTS: Pancreases of 10-week-old male Wistar rats contained 2.8 +/- 0.2 x 10(6) beta cells, of which >90% was generated postnatally, involving: (1) neo-formation of 30,000 aggregates with diameter 200 mum, their beta cells exhibited a higher basal insulin content that was also resistant to glibenclamide-induced degranulation. The pool of Ki67-positive beta cells was sixfold larger than at birth and distributed over all aggregate sizes.
CONCLUSIONS/INTERPRETATION: We describe a method for in situ counting of beta cell numbers and subpopulations with low relative error. In adult rats, >90% of beta cells and beta cell aggregates are formed after birth. Aggregates 200 mum, which are selected for isolated islet studies. Their topographic and functional properties contribute to the functional heterogeneity of the beta cell population; their growth to larger aggregates with characteristic beta cell functions may serve future metabolic needs.
Tijdschrift: Diabetologia
ISSN: 0012-186X
Volume: 53
Pagina's: 2380-2388
Jaar van publicatie:2010
Trefwoorden:endocrine pancreas, heterogeneity, insulin, islets