Asssessment of the in intrinsic pluripotency of mesoderm-derived stem cells from different niches.

During the last decade, human adult stem cells have become an attractive cell source for tissue engineering and for the development of human-relevant alternative in vitro toxicity models. However, distinct stem cell populations show differences in function and differentiation potential. Whether those differences are the result of cell culture, donor variability, or intrinsic properties remains unclear. Therefore, comparative transcriptome analyses were used to determine which of the commonly used human mesoderm-derived stem cell populations, obtained from four distinct niches, displays the highest intrinsic cell plasticity compared to human
embryonic stem cells (hESC): adipose-tissue derived stromal cells (hADSC), bone marrow-derived stromal cells (hBMSC), skin-derived precursor cells (hSKP), or Wharton's jelly-derived mesenchymal stem cells (hWJ). Our data suggest that, compared to hESC gene expression profiles, the intrinsic cell plasticity, defined by
the expression of pluripotency genes and enrichment of biological functions that are involved in embryogenesis and organogenesis, is least prominent in hADSC and most prominent in hSKP, clearly indicating the high multipotent character of the latter.

Jaar van publicatie:2012

Trefwoorden:bone marrow stromal cells, skin-derived precursor