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Exogenous administration of recombinant human FSH does not improve germ cell survival in human prepubertal xenografts.

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In our previous study meiotic activity was observed in human intratesticular xenografts from peripubertal patients. This study aimed to evaluate whether the administration of rhFSH could improve the spermatogonial survival and the establishment of full spermatogenesis in intratesticular human xenografts. Human testicular tissue was obtained from six boys (aged 2.5-12.5 years). The testicular biopsy was fragmented and one fragment of 1.5-3.0 mm3 was transplanted to the testis of immunodeficient nude mice. Transplanted mice were assigned to different experimental groups to enable evaluation of the effects of the administration of rhFSH and freezing. The structural integrity of the seminiferous tubules, the spermatogonial survival and the presence of differentiated cells was evaluated by histology and immunohistochemistry. Freezing or administration of rhFSH did not influence tubule integrity and germ cells survival in human xenografts. Meiotic germ cells were observed in the xenografts. More tubules containing only Sertoli cells (SCO-tubules) were observed in frozen-thawed grafts and more tubules with meiotic cells were present in fresh grafts. In the FSH-treated group less tubules contained meiotic cells and more SCO-tubules were observed.
Administration of rhFSH did not improve the establishment of full spermatogenesis after intratesticular tissue grafting.
Tijdschrift: Reproductive Biomedicine Online
ISSN: 1472-6483
Volume: 26
Pagina's: 286-298
Jaar van publicatie:2013
Trefwoorden:FSH, graft, testis, fertility preservation
  • ORCID: /0000-0001-7601-9689/work/71094722
  • ORCID: /0000-0002-9169-548X/work/61469228
  • ORCID: /0000-0002-0860-1383/work/61279055
  • Scopus Id: 84875368315