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The generation and use of allergen-specific TCR transgenic animals

Boekbijdrage - Hoofdstuk

The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.
Boek: Type 2 immunity : methods and protocols
Series: Methods in Molecular Biology
Pagina's: 183 - 210
ISBN:9781493978960
Jaar van publicatie:2018
Toegankelijkheid:Closed