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CE analysis of U+03B3-globulin mobility and potential clinical utility

Boekbijdrage - Hoofdstuk

Ondertitel:CE analysis of gamma-globulin mobility and potential clinical utility
Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the g-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins were purified from serum using protein l-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the g-globulins (r = 0.2U+2013 0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test.
Boek: Clinical applications of capillary electrophoresis : methods and protocols
Series: Methods in Molecular Biology
Pagina's: 249 - 257
ISBN:9781627030281
Toegankelijkheid:Closed