Screening methods for compounds modulating polyamine transport KU Leuven
Patrizia Agostinis, Wim Annaert, Veerle Baekelandt, Jan Eggermont, Jan Pascal Kahler, Shaun Martin, Johan Swinnen, Chris Van den Haute, Peter Vangheluwe, Roeland Vanhoutte, Steven Verhelst, Sarah van Veen
The invention relates to vitro methods of identifying compounds modulating (stimulating) polyamine transport into a cell comprising the step of: a) providing a first eukaryotic cell culture wherein the endogenous P5B type ATPase gene is inactivated, wherein the cells comprise an inserted copy of a wild type or a disease causing mutant of the P5B type ATPase, and providing a second eukaryotic cell culture wherein the endogenous P5B type ATPase gene is inactivated, wherein the cells comprise an inserted copy of an inactive P5B type ATPase, b) contacting the first and the second cell culture ...