Towards cure of heart disease associated with ryanodine channel 2 Vrije Universiteit Brussel
The objective of the PhD project was to determine the high-resolution structure of cardiacRyR (RyR2) using single particle cryo-EM. In my PhD work, I first developed a purification strategy for RyR1, reconstituted the ion channel into lipid nanodiscs and performed biochemical and structural characterization of RyR1 in a lipid environment. The structure of reconstituted RyR1 was determined under two conditions that revealed an open channel ...