Publications
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[18F]FDG Labeling of neural stem cells for in vivo cell tracking with positron emission tomography: inhibition of tracer release by phloretin Ghent University
The introduction of neural stem cells into the brain has promising therapeutic potential for the treatment of neurodegenerative diseases. To monitor the cellular replacement therapy, that is, to determine stem cell migration, survival, and differentiation, in vivo tracking methods are needed. Ideally, these tracking methods are noninvasive. Noninvasive tracking methods that have been successfully used for the visualization of blood-derived ...
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells Vrije Universiteit Brussel
Abstract
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative ...
Resident and bone marrow-derived mesenchymal stem cells in head and neck squamous cell carcinoma Ghent University
Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers Ghent University
Major depletion of SOX2(+) stem cells in the adult pituitary is not restored which does not affect hormonal cell homeostasis and remodelling KU Leuven
The pituitary gland contains SOX2-expressing stem cells. However, their functional significance remains largely unmapped. We investigated their importance by depleting SOX2+ cells through diphtheria toxin (DT)-mediated ablation. DT treatment of adult Sox2CreERT2/+;R26iDTR/+ mice (after tamoxifen-induced expression of DT receptor in SOX2+ cells) resulted in 80% obliteration of SOX2+ cells in the endocrine pituitary, coinciding with reduced ...
Cell Therapy for Kidney Injury: Different Options and Mechanisms - Mesenchymal and Amniotic Fluid Stem Cells KU Leuven
BACKGROUND: Acute kidney injury (AKI) is emerging as a public health problem in developing and developed countries. It affects up to 7% of hospitalized patients, with a higher prevalence in critical care units. Despite major advances in preventive strategies and support measures, the mortality rate among patients remains higher than 50%. Several pharmacological approaches to improve renal function and survival after an AKI episode have been ...
Stem Cell Microvesicles Transfer Cystinosin to Human Cystinotic Cells and Reduce Cystine Accumulation In Vitro KU Leuven
Cystinosis is a rare disease caused by homozygous mutations of the CTNS gene, encoding a cystine efflux channel in the lysosomal membrane. In Ctns knockout mice, the pathologic intralysosomal accumulation of cystine that drives progressive organ damage can be reversed by infusion of wildtype bone marrow-derived stem cells, but the mechanism involved is unclear since the exogeneous stem cells are rarely integrated into renal tubules. Here we show ...
Manipulating cell fate in mouse embryos and embryonic stem cells Ghent University
Complex signaling networks control all biological processes, including embryogenesis and maintenance of pluripotency in embryonic stem cells (ESCs). Feedbacks and cross talks between transcriptional factors in the network regulate the lineage segregation events during pre-implantation development as well as ESC pluripotency. Small molecules can activate or inhibit certain signaling pathway(s), thereby modulating the fate decisions of these ...