Exploring the scope of nickel pincer as enzymatic cofactor: engineering of gluconate epimerase towards the production of rare sugars. Ghent University
Cofactors provide chemical versatility well beyond the capabilities of enzymes, emphasizing their
potential for biocatalysis. Recently, a tethered niacin-derived nickel pincer cofactor (NPN) has
been discovered in the catalytic site of a lactate racemase for the interconversion of D- and Llactic
acid. Sequence analysis indicated that this fascinating novel cofactor could be widespread in
the prokaryotic world, but has ...