Projects
Real-time qPCR platform enhancing research activities within the Laboratory of Experimental Oncology and the Laboratory of Experimental Radiotherapy KU Leuven
qPCR for gene expression analyses Ghent University
QPCR is one of the basic techniques to investigate gene expression. With this application, we aim at replacing the current machine which is already in shared use for 8 years by the research groups of professors Tina Kyndt, and Godelieve Gheysen (FBE). The current machine is used on a daily basis, by all researchers in both teams, but is currently suffering from technical issues. The qPCR machine that we are applying for here would consist of ...
qPCR system for NGS library quality control, gene expression measurement, hybridization curve, and melting curve analysis Ghent University
In the UGent NXTGNT NGS sequencing laboratory, the Roche LightCycler 480 II qPCR system has served dozens of UGent researchers across several faculties, contributing to hundreds of publications. Also, dozens of external universities, institutions, and commercial partners have benefitted from the system. Most of these researchers indirectly used the system by performing NGS library preparation in the NXTGNT lab. The qPCR system performs ...
A comprehensive evaluation of qPCR and antibody-ELISA to assess the interruption of transmission of soil-transmitted helminth infections Ghent University
Large-scale deworming programs against soil-transmitted helminths (STH) are moving from morbidity control towards interruption of STH transmission. However, there are currently few diagnostic tools that may reliably inform the program when to stop the administration of drugs. We will evaluate qPCR and anti-body ELISAs as diagnostic tools to assess the interruption of STH transmission.
Insights into viral and bacterial colonisation of the lower respiratory tract in cystic fibrosis patients through metagenomic sequencing and multiplex qPCR. University of Antwerp
High-throughput multiplex quantitative PCR KU Leuven
Statistical analysis of real time PCR data Ghent University
Current methdology for the analysis of real time PCR data is incapable to cope with the problems of inhibition and uncertaintiy in the field of GMO detection (food safety). This project focuses on the amelioration of qPCR data analysis by developing a new model for reaction kinetic and measurement uncertainty.
Improving multiplex digital PCR based nucleic acid quantification using data driven analysis tools Ghent University
Digital PCR allows higher order multiplexing compared to real time PCR, enabling simultaneous quantification of multiple targets for single cell research and for diagnosis of cancer mutations or infectious agents. These applications require an appropriate data analysis pipeline. We will tackle this unexplored field by designing 16-plex reactions for single cell analysis of macrophages and mutation detection in cancer.
6-color digital PCR instrument Ghent University
Digital PCR provides superior precision, resolution, and sensitivity for nucleic acid quantification. Since the commercialization of the first digital PCR platform a decade ago, we have established a successful interfaculty digital PCR consortium that places Ghent University as an internationally recognized frontrunner. Over the past year, a new array of next generation digital PCR platforms became available that allow for a much higher ...