Projects
An integrated immunological, proteomic and transcriptomic approach towards the development of an Ascaris vaccine. Ghent University
Round worms (Ascaris spp.) are universal parasites of the small intestines of humans and pigs. The scientific objectives of this proposal are (1) to provide a detailed definition of the nature of the protective mucosal response to the invading L3 larvae and (2) identify the parasite antigens driving this response.
Granzyme-mediated cell-targeted and antiviral methods-of-action: a targeted proteomic analysis Ghent University
The COFRADIC or 'COmbined FRActional DIagonal Chromatography' technology specifically isolates peptides holding the amino terminal part of proteins. Since protein processing gives rise to a new protein fragment, isolation of N-terminal peptides points to proteolytic event and permit analysis of protein processing on a proteome-wide scale. The proteome-wide degradomics methods will be applied to study granzyme-mediated target and host-virus ...
The influence of protease targeting drug leads on substrate processing characte rized in complex proteomes KU Leuven
Proteomes screening for protein fragments which can be secreted by yeast Ghent University
Several decades after the introduction of recombinant DNA technologies for heterologous protein
expression, it remains a matter of trial and error whether a given new protein or protein fragment will
express well in any given expression host cell.
In this project, we aim at developing a novel technology to enable the proteome-wide determination of
which soluble protein fragments can be produced in the yeast secretory ...
Screening entire proteomes for yeast-expressable fragments Ghent University
It is still at present largely unpredictable whether a given protein can be expressed in any production system, including the eukaryotic secretory system. Therefore, we would like to develop a proteome-wide protein fragment "expressability" screening technology in yeast, and manipulate the secretory system to analyze the effect on the "expressable proteome".