The cellenONE instrument with the proteoCHIP technology allows for accurate cell sorting and dispensing, and nanoliter-scale, low-loss automated sample preparation for single-cell mass spectrometry-based proteomics. This instrument will be placed in front of one of our high-end mass spectrometers.
This application is situated in the field of mass spectrometry-based (MS) proteomics. Our consortium brings together unique N-terminomics technologies from the Gevaert lab with the expertise on histone epigenetics and on new Q-TOF acquisition strategies from ProGenTomics (Prof. Deforce and Dr. Dhaenens), the machine learning based identification algorithms developed at the Martens lab and statistical solutions for robust quantification and ...
Mass Spectrometry Imaging (MSI) is an explorative scan technique to analyze chemical compounds within biological tissues. It uses laser beams at multiple (x,y)-coordinates to ionize the molecules at those grid points and measure their mass-to-charge (m/z) ratio. This results in very large data cubes with dimensions x, y, and m/z. Nowadays, the data occupies already tens of gigabytes of memory, and with increasing resolution of the scan ...
The aim of this project is the development of an easily applicable, chemical cleavage protocol using MOFs as artificial proteases for middle down proteomics applications. Metal oxide frameworks (MOFs) are heterogenous catalysts which are robust, cost-effective, and easily recyclable. Selective hydrolysis is of major importance in the field of proteomics and analytical biochemistry, although the hydrolysis of the peptide bond under ...
This project is to seek for a new method of heating milliliter solutions by microwaves. The technology is approached by crossing several research domains: electromagnetic theory, computational electromagnetics, group representation theory, 3D printing of ceramics and metals, and design of thermal metamaterials. We aim to realize a microwave system integrated with the current optical platform, transform the developed system to real-life ...
Olink's groundbreaking proteomics panels help scientists discover human protein biomarkers. Proximity Extension Assay (PEA) technology measures up to 1463 unique proteins in 88 samples simultaneously, starting from less than 3 microliters from nearly any type of biological sample. Unique application domains are located where mass spectrometry (MS) based proteomics is suboptimal. Samples such as cerebrospinal fluid (CSF), microdialysis ...
Complete annotation of the genome is imperative for understanding development, health, and disease. Nevertheless, the annotation of the protein coding genes is far from complete. Especially micropeptides, small proteins less than 100 amino acids, are historically underrepresented in gene annotation databases. In my project proposal, I will develop a machine learning based algorithm to discover novel micropeptides in long non-coding RNA and ...
Breast cancer remains the most frequent malignancy in women and is characterized by complex underlying molecular mechanisms. While major steps have been taken to manage the disease, patients still die, explaining why more research is needed to better characterize, diagnose and treat patients with tailored-made approaches. Defining the right pathological diagnosis remains a key feature in this process as it largely defines the right prognosis ...
The total number of different proteins present in Earth organisms is estimated to be up to 10 million. While their identification in terms of primary sequences is expanding at an increasing rate, elucidation of their function and three-dimensional structure is drastically lacking behind. Most of the currently used approaches in proteomics for unraveling the structure and function of proteins require protein hydrolysis into more manageable ...