Superparamagnetic nanoparticles based isolation of subcellular compartments as a method to identify spatial alterations in protein and lipid composition KU Leuven
Summary
The more than ~25,000 human genes give rise to many more proteins, a.o. by alternative splicing. Genome-wide approaches using database annotations attempt to reveal patterns of protein interactions, however generally ignore the protein locations. Mass spectrometry handles a huge number of proteins ranging widely in abundance, yet its resolution can be enhanced by analyzing purified organellar fractions. This proposal aims to ...