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Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology Ghent University
Vapor nanobubble-mediated photoporation has evolved into a promising physical intracellular delivery technology. When irradiated with short but intense laser pulses, photothermal nanomaterials can generate vapor nanobubbles that, when they collapse, induce transient membrane pores through which exogenous effector molecules can be delivered into the cells. Interestingly, this technique offers high-throughput delivery in various cell types, ...
Surface functionalization with polyethylene glycol and polyethyleneimine improves the performance of graphene-based materials for safe and efficient intracellular delivery by laser-induced photoporation Ghent University
Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability ...
Gold nanoparticle-mediated photoporation enables delivery of macromolecules over a wide range of molecular weights in human CD4+ T cells Ghent University
The modification of CD4+ T cells with exogenous nucleic acids or proteins is a critical step in several research and therapeutic applications, such as HIV studies and cancer immunotherapies. However, efficient cell transfections are not always easily achieved when working with these primary hard-to-transfect cells. While the modification of T cells is typically performed by viral transduction or electroporation, their use is associated with ...
Targeted perturbation of nuclear envelope integrity with vapor nanobubble-mediated photoporation Ghent University University of Antwerp
Selective labeling of individual neurons in dense cultured networks with nanoparticle-enhanced photoporation Ghent University University of Antwerp
Comparing photoporation and nucleofection for delivery of small interfering RNA to cytotoxic T cells Ghent University
The success of cancer immunotherapy through the adoptive transfer of cytotoxic T lymphocytes (CTLs) is highly dependent on the potency of the elicited anti-tumor responses generated by the transferred cells, which can be hindered by a variety of upregulated immunosuppressive pathways. Downregulation of these pathways in the T cells via RNA interference (RNAi) could significantly boost their capacity to infiltrate tumors, proliferate, persist, ...