Delivering compounds into cells is a ubiquitous requirement for fundamental life science research
and cell-based clinical applications. Since cells are protected from the outside world by their plasma
membrane, it requires sophisticated technology to deliver compounds across this barrier without
causing toxicity. Photoporation is emerging as a powerful technology to achieve exactly this. It relies
on laser illumination of ...
Treatment of cells for cell-based therapies is a newer form of therapy in which cells are injected in
patient in order to prevent or treat illnesses. A well-known example is usage of a cancer patientU+2019
own immune cells to attack the tumor cells. Vapour nanobubble (VNB) photoporation is
particularly promising for ex vivo preparation of cells for cell-based therapies as it typically
provides high efficiency, high ...
Optimizing the laser-induced photoporation technology (expanding the transfection portfolio, including larger nucleic acids and plant cells) and translating it into a disruptive, market-worthy product portfolio (device and reagents) and demonstrating the added value compared to the current state-of-the-art transfection methods adhv some user cases.
- increasing the transfection efficiency of large, (negatively charged) molecules. Here it ...
Delivering macromolecular compounds into living cells in vitro or ex vivo is a common requirement for both fundamental biological research as well as biomedical applications. Examples include the intracellular delivery of gene editing molecules to unravel molecular pathways or to create engineered therapeutic cells. Photoporation, which combines laser irradiation with light-sensitive nanomaterials, is an upcoming physical intracellular ...
Laser-induced photoporation is new technology to efficiently deliver compounds into living cells. Here we address the challenge to photoporate cells without the sensitizing nanoparticles being in direct contact with the cell membrane in order to minimize cell toxicity. The technical realization is based on microfluidics, starting with computer simulations followed by the construction and evaluation of dedicated microfluidic chips.
Intracellular delivery of high molecular weight biological molecules is a prerequisite both for fundamental cell biological research as well as for the production of engineered therapeutic cells. This project aims at substantially improving this process for hard-to-transfect cells by combining the strengths of photoporation and electrophoresis, two unrelated techniques that belong to the respective expertises of both promoters.
Since it became evident that Extracellular Vesicles (EVs) have the ability to functionally exchange
biomolecular signals between cells, interest in these nanosized vesicles has grown exponentially,
leading to the identification of a plethora of physiological processes in which EVs are involved.
Because of their natural function as carriers of macromolecular components, they also have
attracted the interest of drug delivery ...
Intravitreal injection, injection in the gel of the eyeball, is a commonly applied method in the clinic
for delivery of drugs (e.g. antibodies) to the retina. However, larger structures like gene carriers
more than often do not reach the retina. This is unfortunate since many blinding diseases require
delivery to this target site. It has been shown that the inner limiting membrane (ILM), a thin
membrane between the ...
Introduction of exogenous material into the cytoplasm of living cells is an important aspect of different biomedical applications. Throughout the cell membrane, inducing toxicity without cell is the most important bottleneck. Meanwhile, various techniques have been developed where electroporation and lipofection are the best known for in vitro and ex vivo applications. Nevertheless, these technologies have important limitations in terms of ...