Photophysics and application of novel fluorescent proteins (FPs) and FP based calcium indicators. KU Leuven
Fluorescent proteins (FPs) are a class of proteins that possess the remarkable feature that they can autocatalytically form the fluorophore after expression from their respective gene, so the fluorescence is genetically encoded. This property, in combination with the existing knowledge of DNA manipulation, allows one to label whichever protein in the genomewith intrinsic fluorescence by making a fusion of the gene coding for the FP with the one ...