Purification of the Secretory Pathway Ca2+/Mn2+ ATPases for Functional Analysis and Drug Discovery KU Leuven
The organellar P2-type Ca2+-transport ATPases, namely Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) and Secretory Pathway Ca²⁺ ATPase (SPCA), are essential to maintain a low resting cytosolic Ca2+ level. At the same time they load respectively the intracellular endoplasmic reticulum (ER) and Golgi stores with Ca2+. This allows Ca2+ signaling to occur and it supports several organellar processes such as protein folding and maturation. ...