Laser microdissection device for immunofluorescent microscopic evaluation and subsequent isolation or manipulation of single celles in heterogeneous tissues for downstream DNA and RNA sequencing or for life cell imaging Ghent University
Laser microdissection enables the isolation of single cells or homogeneous cell clusters from complex heterogeneous tissues. It enables researchers to combine information on the spatial localization of cells with the downstream analysis of the expression profile of RNA, DNA or proteins. Other single cell isolation technologies rely on suspension based cell isolation, which loses all spatial information. With the current proposal, we want to ...
Damage-associated molecular patterns: are they bona fide immunogenic signals or do they simply assist/augment the antigenicity or cancer cells? Ghent University
A limited set of anticancer treatments (like some chemotherapeutics, gamma irradiation and photodynamic therapy) induce an immunogenic cel death (ICD) associated with emission of 'Dammage-Associated Molecular Patterns' (DAMPS). We want to fully understand the role of calreticulin and ATP in ICD, to identify (new) immunomodulators associated with this cell death subroutine and to analyze the receptors that sese these immunological effectors
To grow or not to grow: activation or repression of early oogenesis in the German cockroach, Blattella germanica. KU Leuven
In this one-year postdoctoral mandate we will examine the role of the dopamine receptor 2 (D2R) in the regulation of early oogenesis in the cockroach, Blattella germanica. Using cell-based functional assays we will characterize the BlageD2R. Based on the research of Bai and Palli (Sci. Rep. 6: 1–10, 2016), we hypothesize that this receptor can be activated by both dopamine and juvenile hormone. In close collaboration with ...
To add or to multiply? An investigation of children's preference for additive or multiplicative relations KU Leuven
Multiplicative reasoning is not only omnipresent in numerous daily life situations, it also plays a critical role in the learning of advanced mathematical topics as well as in other fields of study (Lamon, 1993; Lamon & Lesh, 1992; Lesh, Post, & Behr, 1988; Vergnaud, 1988). For these reasons, the development of multiplicative reasoning has been frequently studied. However, it is not achieved easily. Numerous studies indicated that ...
Modulation of endothelial progenitor cell biology by hepatocyte-specific gene transfer of apolipoprotein A-I or the low density lipoprotein receptor. KU Leuven
Computational probing of replicative stress resistance and induced G-quadruplex resolving processes in embryonic stem or cancer cells Ghent University
Fast dividing embryonic stem and cancer cells are subject to stress during DNA replication. G-quadruplexes (G4s) are DNA secondary structures formed by G-rich regions in the DNA, and have recently been shown to play a key role in embryonic stem cell and cancer cell replicative stress. I will apply in-depth bioinformatics analysis on this subject.
The role of the paraoxonase gene family in obesity and obesity-associated liver disease following exposure to environmental pollutants or medical intervention strategies. University of Antwerp
It is estimated that about half of the stars in our Galaxy are binaries. Hence, the impact of binarity on several aspects of astronomy is fundamental. Despite of its importance, there are many aspects of binary interaction physics we do not understand yet.
In this research we will focus on the formation of chemically peculiar stars known as Barium stars. These stars have an excess of barium on their surface as well as several other ...
Acute-on-chronic liver failure (ACLF): monocyte profiles as a predictor for progression or recovery signatures in patients. KU Leuven
We aim to study the dynamics of the monocyte activation status during the course of ACLF, and assess how this associates with disease outcome. By doing paired transcriptional and functional analysis of monocytes isolated at different time-points form recently diagnoses ACLF patients, we aim to profile survivors and non-survivors.