3D super-resolution correlative light and electron microscopy on a dedicated CLEM microscope and development of an automated data acquisition KU Leuven
Fluorescence microscopy can provide information about location of specific protein molecules, by tagging them with fluorescent moieties, or by labeling them with fluorescent antibodies. However, this information is constraint by the limited resolution of the fluorescence microscope as well as by the absence of cellular context on the nano-scale. Therefore it is necessary to employ correlative light and electron microscopy (CLEM), which ...