From theory to practice: Phasor-based insights into FRET-sensing KU Leuven
In recent years the use of Förster resonance energy transfer (FRET) has diversified to many areas in biochemistry. It is a versatile tool to probe distances on the nanometer scale and as such it is used for biosensors, protein interaction studies and single molecule structure characterisation/dynamics.
A wide range of methodologies are used to probe FRET in a lab. While every method has its own advantages and drawbacks, they are ...