Combining photoporation with electrophoresis for improved transfection of hard-to-transfect cells Ghent University
Intracellular delivery of high molecular weight biological molecules is a prerequisite both for fundamental cell biological research as well as for the production of engineered therapeutic cells. This project aims at substantially improving this process for hard-to-transfect cells by combining the strengths of photoporation and electrophoresis, two unrelated techniques that belong to the respective expertises of both promoters.
Transfection of Theileria parva and the role of genes encoding QP-rich proteins in host-parasite interactions Institute of Tropical Medicine
This proposal aims at developing stable transfection in T. parva through a systematic approach
starting with transient transfection, identification of suitable promoter
regions and the development of suitable selectable markers to be used in
integration plasmids. The use of a new generation transfection device and the
expertise in large sporozoite stabilate production should contribute
Exploring ultrasound mediated mRNA transfection of lymph nodes and ultrasound-induced tumor ablation as a synergistic anticancer strategy. Ghent University
This project combines two recent developments in immunotherapy: dendritic cell (DC)-based vaccination and immunogenic cell death (ICD). For this, we will explore high intensity focused ultrasound (HIFU) as a none-invasive means to damage tumor tissue and induce ICD. The immune response will be boosted by immunotherapy using mRNA-loaded microbubbles for ultrasound-triggered delivery of antigen- and adjuvant-mRNA to DCs in vivo.
Novel cationic polymers for non-viral transfection of RNA Ghent University
Nowadays researchers can make synthetic messenger RNAs (mRNAs) that encode for any desired protein. Additionally, small interfering (si)RNAs have been rationally synthetized that specifically inhibit the expression of a selected protein. However, to translate the huge potential of these synthetic RNAs to real applications, efficient carriers that protect and delivery these RNAs inside the cytoplasm of cells are required. This need has ...
MERKEL CELL CARCINOMA, TOWARDS THE IDENTIFICATION OF NOVEL MOLECULAR TARGETS AND THE ESTABLISHMENT OF A THREE-DIMENSIONAL CELL CULTURE MODEL KU Leuven
Merkel cell carcinoma (MCC) is a rare cutaneous neuroendocrine carcinoma. Nevertheless, its incidence has increased in the last few years and this trend is predicted to persist. Furthermore, with a general mortality rate of 33-46% and a high rate of recurrences, it is one of the most aggressive types of skin cancer. Currently, MCC has two recognized etiologies. Merkel cell polyomavirus (MCPyV) DNA is found clonally integrated in the genome of ...
Boosting the cross-talk between human dendritic cells and natural killer cells by Toll-like receptor-ligand-loaded leukemia cells for optimal anti-leukemia T cell responses. University of Antwerp
Development and optimization of cell culture protocols for studying cell-nanoparticle interactions Ghent University
inorganic and organic materials show unique properties when engineered at the nanoscale,
which opens up a myriad of novel applications. In the context of medicine, organic
nanoparticles (NP) are often applied as carriers for insoluble drugs or macromolecules, such
as nucleic acids (NA). The latter require packaging into a nanocarrier to ...
Exploring the market potential of photoporation as the next generation of intracellular delivery technology for the R&D and cell therapy markets Ghent University
Delivering macromolecular compounds into living cells in vitro or ex vivo is a common requirement for both fundamental biological research as well as biomedical applications. Examples include the intracellular delivery of gene editing molecules to unravel molecular pathways or to create engineered therapeutic cells. Photoporation, which combines laser irradiation with light-sensitive nanomaterials, is an upcoming physical intracellular ...