Projects
Combining photoporation with electrophoresis for improved transfection of hard-to-transfect cells Ghent University
Intracellular delivery of high molecular weight biological molecules is a prerequisite both for fundamental cell biological research as well as for the production of engineered therapeutic cells. This project aims at substantially improving this process for hard-to-transfect cells by combining the strengths of photoporation and electrophoresis, two unrelated techniques that belong to the respective expertises of both promoters.
Replic-ON project – Pioneering the investigation of self-amplifying messenger RNA electroporation for long-lasting ex vivo transfection of immune cells. University of Antwerp
Generation of autologous T cells against acute myeloid leukemia by transduction of hematopoietic stem cells with T cell receptor genes and subsequent differentiation to T cells Ghent University
Hematopoietic stem cells will be transduced to express a T Cell Receptor. This receptor will be optained by immunizing against WT1 tumor antigen. The T cell receptor genes will be cloned from WT1 speciific T cells and subsequently used for transduction of the stem cells. Stem cells will be differentiated in vitro to T cells and functionality will be assayed in an in vivo animal model
Generation of autologous T cells against acute myeloid leukemia by transduction of hematopoietic stem cells with T cell receptor genes and subsequent differentiation to T cells Ghent University
Hematopoietic stem cells will be transduced to express a T Cell Receptor. This receptor will be optained by immunizing against WT1 tumor antigen. The T cell receptor genes will be cloned from WT1 speciific T cells and subsequently used for transduction of the stem cells. Stem cells will be differentiated in vitro to T cells and functionality will be assayed in an in vivo animal model
Electrical impedance spectroscopy and 3D cell cultures as emerging technologies for studying signal transduction pathways: the curious case of cathelicidins KU Leuven
This doctoral dissertation has two important parts. The first part covers experiments on elucidating the signal transduction pathways of recently discovered candidate bioactive peptides. The second part introduces 3D cell culture systems and expands on differential gene expression between 2D and 3D cell cultures using RNA-sequencing.
Bioactive peptides perform countless functions in multicellular organisms as key players in cell-cell ...
Influence of therapy on T cell signal transduction in Th1 and Th2 type diseases. University of Antwerp
Innovation mandate with spin-off: Photoporation for in vitro / ex vivo transfecting of cells Ghent University
Optimizing the laser-induced photoporation technology (expanding the transfection portfolio, including larger nucleic acids and plant cells) and translating it into a disruptive, market-worthy product portfolio (device and reagents) and demonstrating the added value compared to the current state-of-the-art transfection methods adhv some user cases.
- increasing the transfection efficiency of large, (negatively charged) molecules. Here it ...
cGMP signal transduction in microvascular endothelial cells of the pressure overloaded and dysfunctional heart. KU Leuven
High blood pressure is a global problem. Untreated, it leads to decreased function of the heart muscle or heart failure with serious consequences for human health and survival. This is one of the reasons why cardiovascular disease remains the leading cause of death worldwide. Failure of our current therapies emphasizes the need for a better understanding of the mechanisms in the evolution of the disease. There are several factors that play a ...
Improved analysis of microRNA mediated regulation of signal transduction in lymphatic endothelial cells. KU Leuven
This research project tries to effectively optimize the analysis of microRNA-mediated regulation of signal transduction in lymphatic endothelial cells.