Growth, virulence expression and toxin production of Bacillus cereus in the small intestine Ghent University
Taking into account the biodiversity of foodborne B. cereus, information will be gained with regard to growth kinetics, gene expression and toxin production in the gastro-intestinal ecological environment using batch cultures and a Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Detection of toxin production will be elaborated via HPCL-MS, real-time PCR performed for monitoring B. cereus cells and gene expression.