Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy Vrije Universiteit Brussel
Many biomolecular interactions proceed via a short-lived encounter state, consisting of multiple, lowly- populated species invisible to most experimental tech- niques. Recent development of paramagnetic relaxation enhancement (PRE) nuclear magnetic resonance (NMR) spectroscopy has allowed to directly visualize such tran- sient intermediates in a number of protein-protein and protein-DNA complexes. Here we present an analysis of the recently ...