Single-cell DNA and RNA sequencing of human cleavage and blastocyst stage embryos to study genomic instability and its functional impact on early development KU Leuven
The human genome is considered stable throughout development. However, recent analyses of human single cells –particularly of human preimplantation embryos following in vitro fertilization as well as of normal human primary tissue– challenge this dogma. Amazingly little is known about the true rate at which somatic DNA-mutation occurs, the different natures of the acquired mutations, as well as the cellular responses and selection processes ...