Publications
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Application of second-generation sequencing (SGS) and third generation sequencing (TGS) in aquaculture breeding program Ghent University
Assessment of ONT sequencing (MinIon) for plant virus detection and comparison with Illumina-based sequencing. Research Institute for Agriculture, Fisheries and Food
Detection of cytosine methylation in Burkholderia cenocepacia by single- molecule real- time sequencing and whole- genome bisulfite sequencing Ghent University
FROM SEQUENCING READS TO MICROBIAL DIVERSITY: BIOINFORMATIC ALGORITHMS FOR PROCESSING AMPLICON SEQUENCING DATA KU Leuven
The development of high-throughput sequencing technologies has revolutionized the field of microbial ecology by offering a cost-efficient method to assess microbial diversity at an unseen depth using 16S RNA amplicon sequencing approaches. Different preprocessing algorithms need to be performed to obtain a collection of highly reliable sequencing reads, ending with a clustering step to group them into Operational Taxonomic Units (OTUs) However, ...
Quantifying next generation sequencing sample pre-processing bias in HIV-1 complete genome sequencing KU Leuven
Genetic analyses play a central role in infectious disease research. Massively parallelized “mechanical cloning” and sequencing technologies were quickly adopted by HIV researchers in order to broaden the understanding of the clinical importance of minor drug-resistant variants. These efforts have, however, remained largely limited to small genomic regions. The growing need to monitor multiple genome regions for drug resistance testing, as well ...
The Interlaboratory RObustness of Next-generation sequencing (IRON) study: a deep sequencing investigation of TET2, CBL and KRAS mutations by an international consortium involving 10 laboratories KU Leuven
Massively parallel pyrosequencing allows sensitive deep sequencing to detect molecular aberrations. Thus far, data are limited on the technical performance in a clinical diagnostic setting. Here, we investigated as an international consortium the robustness, precision and reproducibility of amplicon next-generation deep sequencing across 10 laboratories in eight countries. In a cohort of 18 chronic myelomonocytic leukemia patients, mutational ...