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The perceived impact of isoniazid resistance on outcome of first-line rifampicin-throughout regimens is largely due to missed rifampicin resistance Institute of Tropical Medicine
Background: Meta-analyses on impact of isoniazid-resistant tuberculosis informed the World Health Organization recommendation of a levofloxacin-strengthened rifampicin-based regimen. We estimated the effect of initial rifampicin resistance (Rr) and/or isoniazid resistance (Hr) on treatment failure or relapse. We also determined the frequency of missed initial and acquired Rr to estimate the impact of true Hr.
Methods: Retrospective ...
Rifampicin drug resistance tests for tuberculosis: challenging the gold standard Institute of Tropical Medicine
Setting:The rapid diagnosis of rifampicin resistance is hampered by reported insufficient specificity of molecular techniques for detection of rpoB mutations. Objective:To document the prevalence and prognostic value of rpoB mutations with unclear phenotypic resistance.Design:Sequencing directly from sputum of first failure or relapse patients without phenotypic selection; comparison of standard re-treatment regimen outcome by mutation. ...
Rifampicin resistance missed in automated liquid culture system for Mycobacterium tuberculosis with specific rpoB-mutations Institute of Tropical Medicine
WHO-endorsed phenotypic drug-susceptibility-testing (DST) methods for Mycobacterium tuberculosis are assumed to be the gold standard for identifying rifampicin (RMP) resistance. However, previous results indicate that low-level - yet probably clinically relevant - RMP resistance linked to specific rpoB mutations is easily missed by some growth-based methods. We aimed to compare the level of resistance on Lowenstein-Jensen (LJ) medium with ...
Rifampicin mono-resistant Mycobacterium tuberculosis in Bujumbura, Burundi; results of a drug resistance survey Institute of Tropical Medicine
Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis Institute of Tropical Medicine
Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay Institute of Tropical Medicine
Investigation of rifampicin resistance mechanisms in Brucella abortus using MS-driven comparative proteomics Ghent University
Low cycle threshold value in Xpert MTB/RIF assay may herald false detection of tuberculosis and rifampicin resistance University of Antwerp Institute of Tropical Medicine
We report 2 cases for whom Xpert MTB/RIF falsely signaled rifampicin-resistant tuberculosis, based on unusually low cycle threshold and 3 of 5 probes missing. Other mycobacterial tests were negative. Further optimization of the Xpert MTB/RIF algorithm is warranted.