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Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase Ghent University
Food texture can be improved by enzyme-mediated covalent cross-linking of different food components, such as proteins and carbohydrates. Cross-linking changes the biological and immunological properties of proteins and may change the sensitizing potential of food allergens. In this study we applied a microbial polyphenol oxidase, laccase, to cross-link peanut proteins. The size and morphology of the obtained cross-linked proteins were analyzed ...
The unexpected structure of the designed protein Octarellin V.1 forms a challenge for protein structure prediction tools Vrije Universiteit Brussel
Despite impressive successes in protein design, designing a well-folded protein of more 100 amino acids de novo remains a formidable challenge. Exploiting the promising biophysical features of the artificial protein Octarellin V, we improved this protein by directed evolution, thus creating a more stable and soluble protein: Octarellin V.1. Next, we obtained crystals of Octarellin V.1 in complex with crystallization chaperons and determined the ...
Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery KU Leuven
The Transmembrane Bax Inhibitor-1 motif (TMBIM)-containing protein family is evolutionarily conserved and has been implicated in cell death susceptibility. The only member with a mitochondrial localization is TMBIM5 (also known as GHITM or MICS1), which affects cristae organization and associates with the Parkinson's disease-associated protein CHCHD2 in the inner mitochondrial membrane. We here used CRISPR-Cas9-mediated knockout HAP1 cells to ...
Structure and activity of a cytosolic ribosome-inactivating protein from rice Ghent University
Ribosome-inactivating proteins (RIPs) are cytotoxic enzymes that inhibit protein translation by depurinating ribosomal RNA. Although most plant RIPs are synthesized with leader sequences that sequester them away from the host ribosomes, several RIPs from cereals lack these signal peptides and therefore probably reside in the cytosol near the plant ribosomes. More than 30 RIP genes have been identified in the rice (Oryza sativa spp. japonica) ...
Folding of the MS2 coat protein in Escherichia coli is modulated by translational pauses resulting from mRNA secondary structure and codon usage Research Institute for Agriculture, Fisheries and Food
Possible translational pauses within the coat protein of the RNA bacteriophage MS2 were located on the basis of a distribution plot of rare codons and RNA secondary structure. It appeared that the position of certain codon pauses corresponds with the size of some nascent polypeptide intermediates, which have been isolated from MS2-infected cells. Other accumulated polypeptide intermediates seemed to be related to RNA regions, where ...
Protein Structure Validation Using Side-Chain Chemical Shifts Vrije Universiteit Brussel
We present a method of assessing the quality of protein structures based on the use of side-chain NMR chemical shifts. Because these parameters are very accurate reporters of side-chain positions and are highly sensitive to tertiary structure and packing, they are particularly useful for structure validation. To analyze a given structure, we define a quality score, Q(CS), that compares the chemical shifts calculated from such a structure with ...
Protein structure as a means to triage proposed PTM sites Ghent University
Resolution of protein structure by mass spectrometry Ghent University KU Leuven
High-resolution structure of a papaya plant-defense barwin-like protein solved by in-house sulfur-SAD phasing Vrije Universiteit Brussel
The first crystal structure of a barwin-like protein, named carwin, has been determined at high resolution by single-wavelength anomalous diffraction (SAD) phasing using the six intrinsic S atoms present in the protein. The barwin-like protein was purified from Carica papaya latex and crystallized in the orthorhombic space group P212121. Using in-house Cu Kα X-ray radiation, 16 cumulative diffraction data sets were acquired to increase the ...