Publications
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Protein functionalization revised: NU+2010tertU+2010butoxycarbonylation as an elegant tool to circumvent protein crosslinking Ghent University
In situ monitoring and optimization of CuAAC-mediated protein functionalization of biosurfaces Hasselt University
With the current trend to miniaturize bioactive surfaces to micro-or nanometer scale, the strategy of immobilization becomes more important. Therefore, there is a growing need for protein immobilization techniques that create both stable and homogeneously covered surfaces in a reproducible way. One of the most promising methods to achieve this is the combination of biological receptors with 'click' chemistry, like the Copper catalyzed Alkyne ...
Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation Vrije Universiteit Brussel
An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne functionality at their C-terminus via the intein-mediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle® T7 cells, in the commonly used Luria-Bertani (LB) medium. The combination of these factors results in a ...
Site-selective functionalization of nanobodies using intein-mediated protein ligation for innovative bioconjugation Hasselt University
An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne function at their C-terminus via the Intein-mediated Protein Ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle® T7 cells, in the commonly used Luria-Bertani (LB) medium. The combination of these factors results in a high ...
Plasma functionalization of polycaprolactone nanofibers changes protein interactions with cells, resulting in increased cell viability Ghent University
The surface properties of electrospun scaffolds can greatly influence protein adsorption and, thus, strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen, and ammonia and helium plasma-functionalized polycaprolactone (PCL) nanofibers (NFs) and their cellular interactions by examining the protein corona patterns of the plasma-treated NFs as well as ...
Functionalization of acrylic hydrogels with U+03B1-, U+03B2- or U+03B3-cyclodextrin modulates protein adsorption and antifungal delivery Ghent University
Rational Design and Functionalization of an Artificial Scaffold Protein for Synthetic Biochemistry KU Leuven
Despite remarkable advancements in computational techniques, designing a functional protein from scratch remains very challenging and led scientists to adopt a `bottom-up' approach. Here, existing proteins are used as building blocks for functionalization with active sites or binding motifs. Symmetric globular proteins stand out due to their inherent stability and compatibility with macromolecular assemblies. This thesis first describes two ...