Publications
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Protein Domains as Information Processing Units Vrije Universiteit Brussel
Transducing environmental signals from the cell surface to the nucleus in order to evoke appropriate gene regulatory response requires an accurate and robust medium to propagate biological information. The structure of proteins and especially the dynamic properties of these structures allows for the uptake and restitution of biological information from and to the environment. To understand the functioning and regulation of signalling pathways we ...
Viral macro domains reverse protein ADP-ribosylation KU Leuven
ADP-ribosylation is a post-translational protein modification in which ADP-ribose is transferred from nicotinamide dinucleotide (NAD(+)) to specific acceptors to regulate a wide variety of cellular processes. The Macro domain is an ancient and highly evolutionary conserved protein domain widely distributed throughout all kingdoms of life, including viruses. The human TARG1/C6orf130, MacroD1 and MacroD2 proteins can reverse ADP-ribosylation by ...
Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus Ghent University University of Antwerp
Scavenger receptor CD163 is a key entry mediator for porcine reproductive and respiratory syndrome virus (PRRSV). To identify the CD163 protein domains involved in PRRSV infection, deletion mutants and chimeric mutants were created. Infection experiments revealed that scavenger receptor cysteine-rich (SRCR) domain 5 (SRCR 5) is essential for PRRSV infection, while the four N-terminal SRCR domains and the cytoplasmic tail are not required. The ...
Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface KU Leuven
In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein ...
Structure and Function of the Two Tandem WW Domains of the Pre-mRNA Splicing Factor FBP21 (Formin-binding Protein 21) KU Leuven
Human FBP21 (formin-binding protein 21) contains a matrin-type zinc finger and two tandem WW domains. It is a component of the spliceosomes and interacts with several established splicing factors. Here we demonstrate for the first time that FBP21 is an activator of pre-mRNA splicing in vivo and that its splicing activation function and interaction with the splicing factor SIPP1 (splicing factor that interacts with PQBP1 and PP1) are both ...
Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein. Flanders Institute for Biotechnology Ghent University
Interaction patches of Procaspase-1 Caspase Recruitment Domains (CARDs) are differently involved in Procaspase-1 Activation and Receptor-interacting Protein 2 (RIP2)-dependent nuclear factor κB signaling University of Antwerp Ghent University
Background: The CARD of procaspase-1 regulates activation of procaspase-1 and NF-kappa B. Results: We show that the CARD procaspase-1 interacts with ASC through a type I interaction, whereas RIP2-mediated NF-kappa B activation involves a type III interaction. Conclusion: These findings indicate that the CARD of procaspase-1 is differently involved in inflammasome formation and NF-kappa B activation. Significance: These findings may allow ...
EXPLORING THE ROLE OF PERIPHERAL MYELIN PROTEIN 22 EXPRESSION IN ORDERED MEMBRANE DOMAINS OF SCHWANN CELL DIFFERENTIATED DENTAL PULP STEM CELLS AND HOW IT AFFECTS CELL-MATRIX INTERACTIONS Hasselt University
New therapeutic strategies to establish repair of injured peripheral nerves are desperately needed. For this purpose, our group developed Engineered Neural Tissue with Schwann cell differentiated human dental pulp stem cells (SC-hDPSC) embedded in an aligned collagen hydrogel 1, 2. The use of SC-DPSCs as a cell source for nerve repair has several advantages but there is a need to better understand how they interact with the extracellular matrix ...
Identification and Characterization of Protein Complexes from Total Brain and Synaptoneurosomes: Heterogeneity of Molecular Complexes in Distinct Subcellular Domains KU Leuven
Neurons are highly polarized cells characterized by subcellular microdomains: the synapses. These compartments are specialized structures and are, for certain cellular pathways, independent from the cell body. To achieve such a functional specificity, including local mRNA translation, different molecular complexes are transported along the dendrites and locally regulated. Characterization of such a molecular diversity may help to elucidate ...