Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid Institute of Tropical Medicine
Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.
Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.
Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV ...