Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid KU Leuven University of Antwerp Institute of Tropical Medicine
Evaluation of a new multiplex polymerase chain reaction assay STDFinder for the simultaneous detection of 7 sexually transmitted disease pathogens Institute of Tropical Medicine Ghent University
Rapid detection of Mycobacterium tuberculosis strains resistant to isoniazid and/or rifampicin: standardization of multiplex polymerase chain reaction analysis Institute of Tropical Medicine University of Antwerp
Drug susceptibility testing using molecular techniques can enhance the identification of drug-resistant Mycobacterium tuberculosis. Two multiplex real-time polymerase chain reaction (qPCR) assays were developed to detect the most common resistance-associated mutations to isoniazid (katGS315T, inhA-15C -> T), and rifampicin (rpoBH526Y and rpoBS531L). To assess the species specificity of the qPCR, we selected 31 nontuberculous mycobacteria ...
Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid Institute of Tropical Medicine
Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.
Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.
Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV ...
Diagnostic comparison of Baermann funnel, Koga agar plate culture and polymerase chain reaction for detection of human Strongyloides stercoralis infection in Maluku, Indonesia Institute of Tropical Medicine
Human infection with the nematode Strongyloides stercoralis, which may have a life-threatening course, primarily occurs in tropical settings. Epidemiological data on the occurrence of strongyloidiasis are scarce, and microscopic stool-based detection methods are insensitive. Polymerase chain reaction (PCR) assays have been developed, yet conflicting results have been reported. Our goal was to determine whether there was diagnostic agreement ...