Improving multiplex digital PCR based nucleic acid quantification using data driven analysis tools Ghent University
Digital PCR allows higher order multiplexing compared to real time PCR, enabling simultaneous quantification of multiple targets for single cell research and for diagnosis of cancer mutations or infectious agents. These applications require an appropriate data analysis pipeline. We will tackle this unexplored field by designing 16-plex reactions for single cell analysis of macrophages and mutation detection in cancer.