Publications
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Fluorescence in situ hybridization method using peptide nucleic acid probes for rapid detection of Lactobacillus and Gardnerella spp. Ghent University
Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Lactobacillus spp. in milk samples Ghent University
Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads Institute of Tropical Medicine
We have designed a pair of biotinylated peptide nucleic acid (PNA) probes targeting two sequences in 18S rRNA (from the parasite Trypanosoma brucei) at a distance of 191 nt (corresponding to maximum distance of ca. 60 nm) from each other. The PNA probes were individually bound to (strept)avidin-coated fluorescent beads, differing in size and color [green beads (1 microm) and red beads (5.9 microm)], thereby allowing distinct detection of each ...
Hybridisation Potential of 1',3'-Di-O-methylaltropyranoside Nucleic Acids KU Leuven
In further study of our series of six-membered ring-containing nucleic acids, different 1',3'-di-O-methyl altropyranoside nucleoside analogs (DMANA) were synthesized comprising all four base moieties, adenine, cytosine, uracil and guanine. Following assembly into oligonucleotides (ONs), their affinity for natural oligonucleotides was evaluated by thermal denaturation of the respective duplexes. Data were compared with results obtained previously ...
Detection of Staphylococcus aureus in nasal tissue with peptide nucleic acid-fluorescence in situ hybridization Ghent University
Background: Staphylococcus aureus(SA) in the nose can be a simple colonizer but also may create an intramucosal reservoir causing recurrent infections or can be a specific immune modulator through superantigenic mechanisms. Because the colonization rate of SA is high, but immunologic reactions causing chronic disease are less frequent, the purpose of this study Was to identify the presence of intramucosal SA in healthy subjects and in patients ...
Isolation and characterization of low nucleic acid (LNA)-content bacteria Ghent University
Most planktonic bacteria are 'uncultivable' with conventional methods. Flow cytometry (FCM) is one approach that has been taken to study these bacteria. In natural aquatic environments, bacteria with high nucleic acid (HNA) and low nucleic acid (LNA) content are commonly observed with FCM after staining with fluorescent dyes. Although several studies have focused on the relative abundance and in situ activities of these two groups, knowledge on ...
INNOVATIVE NUCLEIC ACID BASED STRATEGIES FOR FIBER OPTIC SURFACE PLASMON RESONANCE BIOSENSOR DESIGN KU Leuven
The diverse information embedded in the specific sequence of DNA molecules has been feeding our understanding and insights in disease heritage, spreading and evolution. Ultrasensitive detection of the DNA molecules themselves is crucial for the early-stage detection of several diseases, as it facilitates the minimization of actual disease burden. Gold standard technologies, such as the polymerase chain reaction (PCR), are mainly restricted to ...
Targeted detection of nucleic acid mutations for improved lung cancer diagnostics in liquid biopsies KU Leuven
In this project we aimed to improve the current molecular diagnostics approaches for lung cancer- related mutation detection in liquid biopsies. Liquid biopsy refers to sampling and analysis of biological fluids—typically blood—and has potential for screening, diagnosis, predicting and monitoring of treatment response. However, current assays based on circulating tumor DNA (DNA released by tumor cells in the blood) suffer from sensitivity issues ...
How does hydroxyl introduction influence the double helical structure: the stabilization of an altritol nucleic acid:ribonucleic acid duplex KU Leuven
Altritol nucleic acids (ANAs) are a promising new tool in the development of artificial small interfering ribonucleic acids (siRNAs) for therapeutical applications. To mimic the siRNA:messenger RNA (mRNA) interactions, the crystal structure of the ANA:RNA construct a(CCGUAAUGCC-P):r(GGCAUUACGG) was determined to 1.96 Å resolution which revealed the hybrid to form an A-type helix. As this A-form is a major requirement in the RNAi process, this ...