Publications
Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection KU Leuven Ghent University
INNOVATIVE NUCLEIC ACID BASED STRATEGIES FOR FIBER OPTIC SURFACE PLASMON RESONANCE BIOSENSOR DESIGN KU Leuven
Detection of pathogen-specific antibodies by loop-mediated isothermal amplification Vrije Universiteit Brussel Ghent University
Loop-mediated isothermal amplification (LAMP) is a method for enzymatically replicating DNA that has great utility for clinical diagnosis at the point of care (POC), given its high sensitivity, specificity, speed, and technical requirements (isothermal conditions). Here, we adapted LAMP for measuring protein analytes by creating a protein-DNA fusion (referred to here as a "LAMPole") that attaches oligonucleotides (LAMP templates) to IgG ...
Singlet oxygen-based photoelectrochemical detection of nucleic acids University of Antwerp
Detection of Staphylococcus aureus in nasal tissue with peptide nucleic acid-fluorescence in situ hybridization Ghent University
Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates Institute of Tropical Medicine
BACKGROUND: Multiplex ligation-dependent probe amplification (MLPA) is a powerful tool to identify genomic polymorphisms. We have previously developed a single nucleotide polymorphism (SNP) and large sequence polymorphisms (LSP)-based MLPA assay using a read out on a liquid bead array to screen for 47 genetic markers in the Mycobacterium tuberculosis genome. In our assay we obtain information regarding the Mycobacterium tuberculosis lineage ...
Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination Institute of Tropical Medicine KU Leuven University of Antwerp
In countries where malaria transmission has decreased substantially, thanks to the scale-up of control interventions, malaria elimination may be feasible. Nevertheless, this goal requires new strategies such as the active detection and treatment of infected individuals. As the detection threshold for the currently used diagnostic methods is 100 parasites/μL, most low-density, asymptomatic infections able to maintain transmission cannot be ...
Alternative sampling specimens for the molecular detection of mpox (formerly monkeypox) virus Institute of Tropical Medicine KU Leuven
BACKGROUND: Mpox (formerly monkeypox) is a viral disease caused by the mpox virus (MPXV), endemic in Central and West Africa and currently causing a global outbreak of international concern. Much remains unknown about sample types most suited for mpox laboratory diagnosis. While it is established that high viral loads can be found in active skin lesions (currently the recommended mpox laboratory confirmation specimen type), WHO mpox testing ...
Stability of gametocyte-specific Pfs25-mRNA in dried blood spots on filter paper subjected to different storage conditions Institute of Tropical Medicine
BACKGROUND: Real-time quantitative nucleic acid sequence-based amplification (QT-NASBA) is a sensitive method for detection of sub-microscopic gametocytaemia by measuring gametocyte-specific mRNA. Performing analysis on fresh whole blood samples is often not feasible in remote and resource-poor areas. Convenient methods for sample storage and transport are urgently needed.
METHODS: Real-time QT-NASBA was performed on whole blood ...