Projects
Functions of NIPP1 in keratinocyte biology and cutaneous wound repair. KU Leuven
The skin forms a protective barrier over the body. The outer skin layer or epidermis mainly consists of keratinocytes that originate from stem cells in the basal layer or the hair shaft bulge. Keratinocytes gradually move up to the surface of the skin, a process that is accompanied by a step-wise differentiation and eventually apoptosis. When damaged, the skin shows a remarkable ability to heal. An important step of the wound healing process ...
Coordination of cell-cycle progression and DNA-damage repair by the protein phosphatase-1 regulator NIPP1 KU Leuven
NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1) coördineert celgroei en DNA-foutenherstel via een inwerking op eiwitfosfatase-1, een enzym dat regulerende fosfaatgroepen verwijdert van talloze eiwitten. Cellen zonder NIPP1 groeien trager en zijn resistent tegen kankerverwekkende chemicaliën. Met dit onderzoeksproject beogen we, met behulp van nieuwe experimentele modellen, de onderliggende werkingsmechanismen te doorgronden. We zullen ...
A common strategy for counteracting antiviral mechanisms in human and mosquito by CHIKV nsP1 Institute of Tropical Medicine
Interference of protein phosphatase PP1 with the DNA-repair capacity KU Leuven
Cells have evolved elaborate sensing and repair mechanisms to deal with various types of DNA damage. We have found that the deletion of NIPP1, a substrate-targeting subunit of protein phosphatase PP1, dramatically increases the DNA-repair capacity. Conversely, the overexpression of PP1-NIPP1 in human cells induces numerous DNA lesions. Here, we propose to study the molecular mechanism(s) by which PP1-NIPP1 reduces the DNA-repair capacity and ...
SUBSTRATE MAPPING AND ACTIVITY REGULATION OF PP1 HOLOENZYMES KU Leuven
Protein phosphatase 1 (PP1) is an essential protein Ser/Thr phosphatase, which regulates various cellular processes in eukaryotic cells. The activity and substrate specificity of PP1 is strictly controlled by associated polypeptides, which are known as Regulatory Interactors of Protein Phosphatase One (RIPPOs). Currently, more than 200 RIPPOs have been described, all acting as subcellular targeting subunits, substrate recruiters, and/or ...