Transcriptomic profiling of amyloid-induced responses in central and enteric neuronal cultures. University of Antwerp
Nanodiamond labelling of neuronal cells for molecular resolution neuronal and network-level imaging University of Antwerp
Development and validation of a bona fide iPSC derived human neuronal infection model to evaluate antivirals targeted against neurotropic viral infections. University of Antwerp
Exploring the role of Aβ, Tau and Tau modulators in inducing neuronal death in a novel humanized mouse model for Alzheimer Disease KU Leuven
Neuronal cell loss is an ultimate hallmark of the many neurodegenerative diseases including Alzheimer’s disease (AD). However, neuronal cell death phenotype is seldom investigated. In AD, the relationship between Amyloid β (Aβ) peptide, Tau, and neuronal death is a central problem. Nevertheless, cell culture or animal models do not reproduce well the cell death phenotype that is crucial to analyze this relation. As it remains unclear to what ...
The relationship between Amyloid ɴ(Aɴpeptide, Tau, and neuronal cell loss is a central problem in the study of Alzheimer’s disease (AD). However, cell culture or animal models do not reproduce well the cell death phenotype that is crucial to analyze this relation. As it remains unclear to what extent rodent or other species can fully reproduce the AD phenotype, such study is performed ideally in human cells. In the current project, we take ...
Development of a human in vitro neuronal model for the MECP2 duplication syndrome using induced pluripotent stem cell technology KU Leuven
The causal relationship between a disease and the underlying
genetic defect are commonly studied in animal models. However, species specific
difference in the genetic, anatomical andphysiological characteristics between
animals and humans present a major barrier in extending
the information to human diseases. Alsoin few instances,
the preclinical treatments that show success in ...
study of the underlying cellular and molecular mechanisms of detrimental ROCK signaling in neuronal survival and axonal outgrowth. KU Leuven
Since the adult mammalian central nervous system (CNS) lacks the capacity to replace lost nerve cells or to regenerate injured axons, neurodegenerative disorders seriously affect life quality and human wellbeing. Understanding the pathophysiological mechanisms underlying neurodegeneration is essential to support and develop novel therapeutic strategies to promote neural repair. Rho kinases (ROCK) regulate pleiotropic cellular functions, but ...
Study of the essential function of beta-actin in embryonic development and the necessity for correct localisation of beta-actin MRNA during neuronal development Ghent University
Through analysis of the B-actin knock-out en knock-in mice, and derived cell lines thereof, we will study the essential functions of B-actin in development (more specific in vascularisation and neuronal differentiation) and the role of the 3'UTR in correct localization and function of B-actin. Several methods will be used, including in situ immunostaining, primary cell cultures, RNAi and differential proteome analysis.
STATE OF THE ART
Reliable and easy accessible experimental models are the key to fruitful translational research. Talking about biomedical research, every scientist in this field would like to have human material at hand to perform their experiments, but this is not always possible. Especially in the field of neuroscience, researchers face the scarce availability of human nerve tissue (which, in fact, can only ...