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Evaluation of the BD MGIT TBc Identification Test (TBc ID), a rapid chromatographic immunoassay for the detection of Mycobacterium tuberculosis complex from liquid culture Institute of Tropical Medicine
The BACTEC MGIT 960 system is increasingly used to culture Mycobacterium tuberculosis. We evaluated the performance of the new immunochromatographic assay BD MGIT TBc Identification Test (TBc ID) for the rapid identification of M. tuberculosis complex in clinical samples when performed directly from BACTEC MGIT 960 culture positive for acid-fast bacilli (AFB). Of 92 cultures evaluated, the sensitivity and specificity of the TBc ID test was 98.5% ...
Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis Institute of Tropical Medicine Ghent University
OBJECTIVES: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting.
METHODS: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were ...
Re-evaluation of current critical concentrations of fourthgeneration anti-TB fluoroquinolones in the MGIT 960 system Institute of Tropical Medicine
Moxifloxacin (Mfx) and gatifloxacin (Gfx) are fourth-generation fluoroquinolones (FQ) that prevent DNA synthesis of Mycobacterium tuberculosis complex (MTBc). The gold standard for FQ resistance testing is phenotypic drug-susceptibility testing (pDST) using drug-containing media at a critical concentration (CC) to differentiate phenotypically wild type and phenotypically non-wild type MTBC, and at a clinical breakpoint to differentiate strains ...
Time to detection of the growth of **Mycobacterium tuberculosis** in MGIT 960 for determining the early bactericidal activity of antituberculosis agents University of Antwerp
Evaluation of early bactericidal activity (EBA) by the determination of a fall in viable colony-forming units (CFU) of Mycobacterium tuberculosis in sputum is a first step in the clinical study of new antituberculosis agents. The time to detection (TTD) of growth in liquid media is more sensitive and could substitute for CFU counting on solid media. Overnight sputum samples collected during the evaluation of the novel agent TMC207 in comparison ...
Mycobacterium tuberculosis pncA polymorphisms that do not confer pyrazinamide resistance at a breakpoint concentration of 100 micrograms per milliliter in MGIT University of Antwerp
Sequencing of the Mycobacterium tuberculosis pncA gene allows for pyrazinamide susceptibility testing. We summarize data on pncA polymorphisms that do not confer resistance at a susceptibility breakpoint of 100 mu g/ml pyrazinamide in MGIT within a cohort of isolates from South Africa and the U.S. Centers for Disease Control and Prevention.