Publications
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Porcine small intestinal organoids as a model to explore ETEC–host interactions in the gut Ghent University
Small intestinal organoids, or enteroids, represent a valuable model to study host-pathogen interactions at the intestinal epithelial surface. Much research has been done on murine and human enteroids, however only a handful studies evaluated the development of enteroids in other species. Porcine enteroid cultures have been described, but little is known about their functional responses to specific pathogens or their associated virulence ...
Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation Ghent University
Intestinal Organoids in Inflammatory Bowel Diseases KU Leuven
Ulcerative colitis (UC) and Crohn’s disease (CD) are the two major forms of inflammatory bowel diseases (IBD), which are chronic and progressive diseases of the gastrointestinal tract. The exact cause is unknown, but it has been suggested that the diseases result from an abnormal immune response towards environmental factors, such as the microbiota, in a genetically predisposed person. Current therapies aim to stop this inflammatory cascade. The ...
Correction of CFTR function in intestinal organoids to guide treatment of cystic fibrosis KU Leuven
RATIONALE: Given the vast number of cystic fibrosis transmembrane conductance regulator (CFTR) mutations, biomarkers predicting benefit from CFTR modulator therapies are needed for subjects with cystic fibrosis (CF). OBJECTIVES: To study CFTR function in organoids of subjects with common and rare CFTR mutations and evaluate correlations between CFTR function and clinical data. METHODS: Intestinal organoids were grown from rectal biopsies in a ...
Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds KU Leuven
Tissue engineering is an interdisciplinary field that combines stem cells and matrices to form functional constructs that can be used to repair damaged tissues or regenerate whole organs. Tissue stem cells can be expanded and functionally differentiated to form 'mini-organs' resembling native tissue architecture and function. The choice of the scaffold is also pivotal to successful tissue reconstruction. Scaffolds may be broadly classified into ...
Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure KU Leuven
Intestinal failure, following extensive anatomical or functional loss of small intestine, has debilitating long-term consequences for children1. The priority of patient care is to increase the length of functional intestine, particularly the jejunum, to promote nutritional independence2. Here we construct autologous jejunal mucosal grafts using biomaterials from pediatric patients and show that patient-derived organoids can be expanded ...
Evaluation of 3D Human Intestinal Organoids as a Platform for EV-A71 Antiviral Drug Discovery KU Leuven
Enteroviruses are a leading cause of upper respiratory tract, gastrointestinal, and neurological infections. Management of enterovirus-related diseases has been hindered by the lack of specific antiviral treatment. The pre-clinical and clinical development of such antivirals has been challenging, calling for novel model systems and strategies to identify suitable pre-clinical candidates. Organoids represent a new and outstanding opportunity to ...