Effects of low methyl donor levels in culture medium during mouse follicle culture on oocyte imprinting establishment. Vrije Universiteit Brussel
Imprinted genes are differentially methylated during gametogenesis to allow parent-of-origin specific monoallelic expression. We previously demonstrated normal imprinting establishment at four key imprinted genes in mouse MII oocytes after in vitro follicle culture. Commercially available culture media feature a wide range of methyl donor levels. The aim was to study the effect of low methyl donor (methionine, vitamin B12, folic acid, choline ...