Publications
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QMS Teicoplanin serum immunoassay performance on the Thermo Scientific Indiko Plus and comparison to fluorescence polarization immunoassay Ghent University
A competitive immunoassay for zearalenone with integrated poly(dimethysiloxane) based microarray assay Ghent University
Multiplex flow-through immunoassay formats for screening of mycotoxins in a variety of food matrices Ghent University
Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins Ghent University
Quantum dot loaded liposomes as fluorescent labels for immunoassay Ghent University
Diagnostic characterization of respiratory allergies by means of a multiplex immunoassay Hasselt University
Allergic sensitization is commonly assessed in patients by performing the skin prick test (SPT) or determining specific immunoglobulin (IgE) levels in blood samples with the ImmunoCAP (TM) assay, which measures each allergen and sample separately. This paper explores the possibility to investigate respiratory allergies with a high throughput method, the Meso Scale Discovery (MSD) multiplex immunoassay, measuring IgE levels in low volumes of ...
An experimental design approach to optimize an amperometric immunoassay on a screen printed electrode for Clostridium tetani antibody determination Vrije Universiteit Brussel
An immunoassay for the determination of anti-tetani antibodies has been developed using a screen printed electrode (SPE) as solid support for toxoid (antigen) immobilization. The assay was performed in guinea pig serum. The immunoreaction and the subsequent amperometric detection occurred directly onto the SPE surface. The assay consisted of spiking the anti-tetani sample directly onto the toxoid modified SPE, and then a second antibody, i.e. a ...
Development of a multipurpose time-resolved fluorescence immunoassay for rat insulin Vrije Universiteit Brussel
We present a time-resolved fluorescence immunoassay (TR-FIA) for the measurement of rat insulin in cell extracts and culture media. This assay is based on the binding of two monoclonal antibodies to different parts of the insulin molecule in a 96-well microtiter plate. For the detection, europium-labeled streptavidin that interacts with the second biotinylated antibody is used. Samples of 25mul could be analyzed in less than 2days with a ...
Quantum dots : gold nanoparticles FRET based system immunoassay Ghent University
Forster resonance energy transfer (FRET) based on colloidal gold nanoparticles (AuNPs) and quantum dots (QDs) was used as a system for detection of deoxynivalenol (DON) mycotoxin in competitive immunoassay. An enhancement of distance between antibody and antigen in the presence of analyte cause decreasing of FRET and increasing of QDs luminescence.