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Comprehensive analysis of the effective and intra-particle diffusion of weakly retained compounds in silica hydrophilic interaction liquid chromatography columns Vrije Universiteit Brussel KU Leuven

Lídia Redón, Xavier Subirats, Soraya Chapel, Timothy Januarius, Ken Broeckhoven, Martí Rosés, Deirdre Cabooter, Gert Desmet

A detailed analysis of intra-particle volumes and layer thicknesses and their effect on the diffusion of solutes in hydrophilic interaction liquid chromatography (HILIC) was made. Pycnometric measurements and the retention volume of deuterated mobile phase constituents (water and acetonitrile) were used to estimate the void volume inside the column, including not only the volume of the mobile phase but also part of the enriched water solvent ...

Metabolic profiling of human plasma and urine in chronic kidney disease by hydrophilic interaction liquid chromatography coupled with time-of-flight mass spectrometry : a pilot study Ghent University

Jente Boelaert, Frederic Lynen, Griet Glorieux, Eva Schepers, Nathalie Neirynck, Raymond Vanholder
A typical characteristic of chronic kidney disease (CKD) is the progressive loss in renal function over a period of months or years with the concomitant accumulation of uremic retention solutes in the body. Known biomarkers for the kidney deterioration, such as serum creatinine or urinary albumin, do not allow effective early detection of CKD, which is essential towards disease management. In this work, a hydrophilic interaction liquid ...

Evaluation and comparison of the kinetic performance of ultra-high performance liquid chromatography and high-performance liquid chromatography columns in hydrophilic interaction and reversed-phase liquid chromatography conditions (vol 1369, pg 83, 2014) Vrije Universiteit Brussel KU Leuven

Huiying Song, Erwin Adams, Gert Desmet, Deirdre Cabooter
An intrinsic performance comparison is made of the reduction in analysis time that can be obtained when switching from HPLC to UHPLC column formats in HILIC and reversed-phase conditions. A detailed overview of the packing characteristics of both stationary phase types is given first. It is demonstrated that HILIC columns demonstrate higher external porosity values than their reversed-phase counterparts resulting in lower flow resistance values. ...

Evaluation and Comparison of the Kinetic Performance of Ultra-High Performance Liquid Chromatography and High-Performance Liquid Chromatography Columns in Hydrophilic Interaction and Reversed-Phase Liquid Chromatography Conditions KU Leuven

Huiying Song, Erwin Adams, Deirdre Cabooter
An intrinsic performance comparison is made of the reduction in analysis time that can be obtained when switching from HPLC to UHPLC column formats in HILIC and reversed-phase conditions. A detailed overview of the packing characteristics of both stationary phase types is given first. It is demonstrated that HILIC columns demonstrate higher external porosity values than their reversed-phase counterparts resulting in lower flow resistance values. ...

Evaluation and comparison of the kinetic performance of ultra-high performance liquid chromatography and high-performance liquid chromatography columns in hydrophilic interaction and reversed-phase liquid chromatography conditions Vrije Universiteit Brussel

Huiying Song, Erwin Adams, Gert Desmet, Deirdre Cabooter
An intrinsic performance comparison is made of the reduction in analysis time that can be obtained when switching from HPLC to UHPLC column formats in HILIC and reversed-phase conditions. A detailed overview of the packing characteristics of both stationary phase types is given first. It is demonstrated that HILIC columns demonstrate higher external porosity values than their reversed-phase counterparts resulting in lower flow resistance values. ...

In-Depth Performance Analysis and Comparison of Monolithic and Particulate Zwitterionic Hydrophilic Interaction Liquid Chromatography Polymer Columns Vrije Universiteit Brussel KU Leuven

Haibin Li, Zhengjin Jiang, Gert Desmet, Deirdre Cabooter

The kinetic performance of different zwitterionic hydrophilic interaction liquid chromatography polymer columns is evaluated and compared in-depth. For this purpose, two lab-made monolithic columns, synthesized with different crosslinkers, and a commercial particle packed column are considered. It is found that performance evaluation techniques, such as comparing plate height curves or fitted A-, B- and C-terms, obtained by fitting ...

Phase Diagram and Structures in Mixtures of Poly(styrenesulfonate anion) and Alkyltrimethylammonium Cations in Water: Significance of Specific Hydrophobic Interaction KU Leuven

Bart Goderis
Mixtures of polyelectrolytes and oppositely charged surfactants show very rich phase behavior that is influenced by surfactant-ion and polyion properties and by water content. A general feature is associative phase separation as a result of strong electrostatic interactions, whereas the effect of eventual more specific interactions (e.g., hydrophobic) has not been thoroughly investigated. In this paper, we report a detailed study on phase ...

High-throughput protein precipitation and hydrophobic interaction chromatography: Salt effects and thermodynamic interrelation KU Leuven

Peter Verhaert
Salt-induced protein precipitation and hydrophobic interaction chromatography (HIC) are two widely used methods for protein purification. In this study, salt effects in protein precipitation and HIC were investigated for a broad combination of proteins, salts and HIC resins. Interrelation between the critical thermodynamic salting out parameters in both techniques was equally investigated. Protein precipitation data were obtained by a ...

Analysis Profiling of 48 Endogenous Amino Acids and Related Compounds in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry KU Leuven

Erwin Adams, Ann Van Schepdael
Analyzing and detecting endogenous amino acids in blood is of crucial importance for the diagnosis of medical conditions and scientific research. Considering the lack of UV chromophores in most of these analytes and the presence of several interfering substances in plasma, the quantification of quite a few amino acids and related compounds presents certain technical challenges. As a blank plasma matrix lacking these endogenous substances does ...

Selectivity and Resolving Power of Hydrophobic Interaction Chromatography Targeting the Separation of Monoclonal Antibody Variants Vrije Universiteit Brussel

Raphael Ewonde Ewonde, Katharina Böttinger, Jelle De Vos, Nico Lingg, Alois Jungbauer, Christopher A. Pohl, Christian G. Huber, Gert Desmet, Sebastiaan Eeltink

This study presents a comprehensive investigation of the mechanistic understanding of retention and selectivity in hydrophobic interaction chromatography. It provides valuable insights into crucial method-development parameters involved in achieving chromatographic resolution for profiling molecular variants of trastuzumab. Retention characteristics have been assessed for three column chemistries, i.e., butyl, alkylamide, and long-stranded ...