Analysis of data obtained by fluorescence fluctuation microscopy from a plurality of dyes Hasselt University
The analysis of data from fluorescence microscopy is disclosed, more especially fluorescence fluctuation microscopy, being a correlation analysis of fluctuation of the fluorescence intensity enabling quantitative and dynamic information capture. In particular the analysis is described of characteristics such as concentrations, mobility, interactions, stoichiometry, etc. of mixtures of particles that are fluorescently labeled with differently colored fluorophores having different excitation/emission spectra using fluctuation microscopy.