Publications
Detection of tuberculosis drug resistance: a comparison by Mycobacterium tuberculosis MLPA assay versus Genotype®MTBDRplus Institute of Tropical Medicine
BACKGROUND: To cope with the emergence of multidrug-resistant tuberculosis (MDR-TB), new molecular methods that can routinely be used to screen for a wide range of drug resistance related genetic markers in the Mycobacterium tuberculosis genome are urgently needed.
OBJECTIVE: To evaluate the performance of multiplex ligaton-dependent probe amplification (MLPA) against Genotype® MTBDRplus to detect resistance to isoniazid (INHr) and ...
Combinatorial drug approaches to tackle Candida albicans biofilms KU Leuven
In vitro activity of the antiasthmatic drug zafirlukast against the oral pathogens Porphyromonas gingivalis and Streptococcus mutans KU Leuven
Drug repurposing: phosphate prodrugs of anticancer and antiviral FDA-approved nucleosides as novel antimicrobials KU Leuven
Global epidemiology of antimicrobial resistance in commensal Neisseria species: a systematic review Institute of Tropical Medicine
BACKGROUND: Commensal Neisseria species (spp). represent an important reservoir of antimicrobial resistance genes for pathogenic Neisseria spp. In this systematic review, we aimed to assess the antimicrobial susceptibility of commensal Neisseria spp. and how this has evolved over time. We also aimed to assess if commensal Neisseria spp. showed intrinsic resistance to four antimicrobials - penicillin, azithromycin, ceftriaxone and ...
Evolution and mechanisms of bacterial persistence, a shortcut for evolving genetic resistance KU Leuven
Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates Institute of Tropical Medicine
BACKGROUND: Multiplex ligation-dependent probe amplification (MLPA) is a powerful tool to identify genomic polymorphisms. We have previously developed a single nucleotide polymorphism (SNP) and large sequence polymorphisms (LSP)-based MLPA assay using a read out on a liquid bead array to screen for 47 genetic markers in the Mycobacterium tuberculosis genome. In our assay we obtain information regarding the Mycobacterium tuberculosis lineage ...
A near-full length genotypic assay for HCV1b Vrije Universiteit Brussel KU Leuven
A near-full genome genotypic assay for HCV1b was developed, which may prove useful to investigate antiviral drug resistance, given new combination therapies for HCV1 infection. The assay consists of three partially overlapping PCRs followed by Sanger population or Illumina next-generation sequencing. Seventy-seven therapy-naïve samples, spanning the entire diversity range of currently known HCV1b, were used for optimization of PCRs, of which ...
Doxycycline Post Exposure Prophylaxis could induce cross-resistance to other classes of antimicrobials in Neisseria gonorrhoeae: an in-silico analysis Institute of Tropical Medicine
We found that tetracycline resistance-associated mutations and genes in Neisseria gonorrhoeae are linked to mutations causing resistance to other antimicrobials. Therefore, the use of doxycycline postexposure prophylaxis may select for resistance to other antimicrobials.