Leveraging droplet microfluidics and nucleic acid engineering for development of a CRISPR-based cell imaging toolbox KU Leuven
Fluorescence in situ hybridization (FISH) is the golden standard for DNA imaging in cells, but it involves lengthy experimental workflows and genomic DNA denaturation. CRISPR-Cas9 (stands for: Clustered Regularly Interspaced Short Palindromic Repeats and associated protein 9) technology, alternatively, has enabled specific cell imaging without the need for DNA denaturation. Still, all so far reported CRISPR-Cas9-based approaches require ...