Towards harmonization of DNA metabarcoding for monitoring marine macrobenthos: The effect of technical replicates and pooled DNA extractions on species detection Research Institute for Agriculture, Fisheries and Food Ghent University
DNA-based monitoring methods are potentially faster and cheaper compared to traditional morphological benthic identification. DNA metabarcoding involves various methodological choices which can introduce bias leading to a different outcome in biodiversity patterns. Therefore, it is important to harmonize DNA metabarcoding protocols to allow comparison across studies and this requires a good understanding of the effect of methodological choices ...