Production, crystallisation and X-ray diffraction analysis of two nanobodies against the Duffy Binding like domain DBL6e-FCR3 of the Plasmodium falciparum VAR2CSA protein Vrije Universiteit Brussel
The VAR2CSA protein has been closely associated with pregnancy-associated malaria and is recognized as the main adhesin exposed on the surface of Plasmodium falciparum-infected erythrocytes. Chondroitin sulfate A was identified as the main host receptor in the placenta. Single-domain heavy-chain camelid antibodies, more commonly called nanobodies, were selected and produced against the DBL6"-FCR3 domain of VAR2CSA. Crystals of two specific ...