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High level of cross-resistance between kanamycin, amikacin, and capreomycin among **Mycobacterium tuberculosis** isolates from Georgia and a close relation with mutations in the rrs gene University of Antwerp
The aminoglycosides kanamycin and amikacin and the macrocyclic peptide capreomycin are key drugs for the treatment of multidrug-resistant tuberculosis (MDR-TB). The increasing rates of resistance to these drugs and the possible cross-resistance between them are concerns for MDR-TB therapy. Mutations in the 16S rRNA gene (rrs) have been associated with resistance to each of the drugs, and mutations of the tlyA gene, which encodes a putative rRNA ...
Development and validation of a liquid chromatographic method for the analysis of capreomycin sulfate and its related substances KU Leuven
A gradient LC method for the analysis of capreomycin sulfate and its related substances was developed. The chromatographic conditions include the use of a Hypersil base deactivated C-18 (250 mm x 4.6 mm, 5 mu m) column maintained at 25 degrees C, a mobile phase containing aceronitrile, phosphate buffer pH 2.3 and 0.025 M hexanesulfonate at a flow rate of 1.0 mL/min and UV detection performed at 268 nm. Good separation of the four active ...
Impurity profiling of capreomycin using dual liquid chromatography coupled to mass spectrometry KU Leuven
The characterization of unknown (UNK) impurities in capreomycin (CMN) using liquid chromatography coupled to mass spectrometry (LC/MS) has been described. The ion-pair liquid chromatography method coupled to ultraviolet detection (LC-UV) described by Mallampati et al. was used for the separation of CMN from its related substances. As the method uses non-volatile reagents it could not be directly coupled to mass spectrometry (MS) for impurity ...
Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis Institute of Tropical Medicine
Objectives To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional ...
Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system Institute of Tropical Medicine Ghent University
Synthesis of potential inhibitors of a novel flavin-dependent thymidylate synthase in Mycobacterium tuberculosis KU Leuven
SummaryHuman tuberculosis is a contagious infectious disease and is regarded as a major threat to global health. It is estimated that one third of the world population is infected with Mycobacterium tuberculos is, the causative agent of this disease. The emergence of drug-resistant strains of M. tuberculosis in synergy with the rise of the HIV/AIDS epi demic has further severely exacerbated effective TB-control. Drug-suscep tible TB is treated ...
Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis Ghent University
Objectives: To perform a multicentre study to evaluate the performance of the colorimetric redox indicator (CRI) assay and to establish the MICs and critical concentrations of rifampicin, isoniazid, ofloxacin, kanamycin and capreomycin. Methods: The study was carried out in two phases. Phase I determined the MIC of each drug. Phase II established critical concentrations for the five drugs tested by the CRI assay compared with the conventional ...
A standardised method for interpreting the association between mutations and phenotypic drug resistance in Mycobacterium tuberculosis Institute of Tropical Medicine
A clear understanding of the genetic basis of antibiotic resistance in Mycobacterium tuberculosis is required to accelerate the development of rapid drug susceptibility testing methods based on genetic sequence.
Raw genotype-phenotype correlation data were extracted as part of a comprehensive systematic review to develop a standardised analytical approach for interpreting resistance associated mutations for rifampicin, isoniazid, ...
Distribution of common and rare genetic markers of second-line-injectable-drug resistance in Mycobacterium tuberculosis revealed by a genome-wide association study University of Antwerp Institute of Tropical Medicine
Point mutations in the rrs gene and the eis promoter are known to confer resistance to the second-line injectable drugs (SLIDs) amikacin (AMK), capreomycin (CAP), and kanamycin (KAN). While mutations in these canonical genes confer the majority of SLID resistance, alternative mechanisms of resistance are not uncommon and threaten effective treatment decisions when using conventional molecular diagnostics. In total, 1,184 clinical Mycobacterium ...