Engineering viral vectors for high-throughput CRISPR genetic screens in plants KU Leuven
Tools based on CRISPR-Cas systems hold great potential both for fundamental plant genetics and precision crop breeding. Unlike traditional mutagenic approaches, CRISPR-Cas systems allow for the simultaneous delivery of thousands of unique guide RNAs, enabling the generation of collections of higher-order mutants for rapid functional screens and the discovery of genetic interactions. However, CRISPR technology suffers from a ‘transformation ...