Publications
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Enhanced phycocyanin and protein content of Arthrospira by applying neutral density and red light shading filters: a small-scale pilot experiment Hasselt University KU Leuven
BACKGROUND: The cyanobacterium Arthrospira contains proteins and the blue pigment phycocyanin that can be used as a natural colourant in the food industry. The aim of this study was to investigate a strategy for increasing the yield of phycocyanin and protein from Arthrospira cultures by shading the cultures with neutral density and red light filters. Experimentswereconductedunder threedifferent irradianceconditions: (i) laboratory testsunder ...
Thermal and acidic denaturation of phycocyanin from Arthrospira platensis: Effects of complexation with λ-carrageenan on blue color stability KU Leuven
The pH and temperature sensitivity of the natural blue pigment phycocyanin from Arthrospira platensis limits its application as food colorant. This study examines the effect of protein stabilization by the anionic polysaccharide λ-carrageenan on phycocyanins color appearance at pH 2.5-6.0, unheated and after heat treatments (70/90 °C). Electrostatic interactions, hydrophobic interactions, hydrogen bonds and disulfide-bridges were assessed by ...
Production of the natural blue dye phycocyanin by the cyanobacterium Arthrospira KU Leuven
Dyes are widely used to colour foodstuffs, cosmetics and textiles. Due to the increasing preference of the consumers to natural over synthetic dyes, sythetic dyes are being replaced by natural ones. Phycocyanin is a blue phycobiliprotein pigment produced commercially from the biomass of the cyanobacterium Arthrospira. It is used as a natural colorant in the food industry (mainly in candies and beverages) and can be potentially used in textile ...
Advancement of green process through microwave-assisted extraction of bioactive metabolites from Arthrospira Platensis and bioactivity evaluation KU Leuven
Bioactivity and functional properties of cyanobacterial extract mostly depends on process of extraction, temperature and solvent used (polar or non-polar). To evaluate these parameters a design of experiment (DOE; using a 2k design) was performed with Arthrospira platensis. Extraction process was optimized through microwave-assisted extraction considering solvent ratio, temperature and time of extraction with polar (PS) and non-polar (NPS). ...
Extending Landsat 8 : retrieval of an orange contra-band for inland water quality applications Ghent University
The Operational Land Imager (OLI) onboard Landsat 8 has found successful application in inland and coastal water remote sensing. Its radiometric specification and high spatial resolution allows quantification of water-leaving radiance while resolving small water bodies. However, its limited multispectral band set restricts the range of water quality parameters that can be retrieved. Identification of cyanobacteria biomass has been demonstrated ...
Covalent binding of food-derived blue pigment phycocyanobilin to bovine β-lactoglobulin under physiological conditions Ghent University
In this study, we investigated structural aspects of covalent binding of food derived blue pigment phycocyanobilin (PCB) to bovine beta-lactoglobulin (BLG), major whey protein, by spectroscopic, electrophoretic, mass spectrometry and computational methods. At physiological pH (7.2), we found that covalent pigment binding via free cysteine residue is slow (k(a)=0.065 min(-1)), of moderate affinity (K-a=4x10(4) M-1), and stereo-selective. Binding ...
Contrasting effects of monochromatic LED lighting on growth, pigments and photosynthesis in the commercially important cyanobacterium Arthrospira maxima Ghent University
The aim of this study was to evaluate the effects of different colored light emitting diodes (LEDs) on the growth, pigment yield, and photosynthetic performance of Arthrospira maxima, a commercially exploited species of cyanobacteria. The highest growth and chlorophyll a (Chl a) concentration were obtained under red LED and white fluorescent light, while the lowest growth and Chl a concentration were observed under blue LED. However, blue LED ...